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269413

Anti-biofilm Effect of Copper Oxide Nanoparticles, EDTA, and Kojic Acid on MDR-biofilm Forming Gram Negative Superbugs in Sohag University Hospitals, Egypt

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

Antimicrobial resistance

Abstract

Background: Multidrug-resistant (MDR) Gram negative superbugs are considered among the most important causes of healthcare associated infections and these resistant isolates are usually present in association with biofilms rather than planktonic existence. Objectives: This study aimed to assess the ability of EDTA, Kojic acid, and CuONPs to inhibit the phenomenon of biofilm formation by multidrug-resistant Gram-negative superbugs. Methodology: Gram negative bacilli were isolated on suitable media and identified biochemically by vitek-2 biochemical identification system. MDR isolates were tested for biofilm formation by tissue culture plate method, biofilm-producing isolates were exposed EDTA and Kojic acid by certain concentration and to copper oxide nanoparticle (CuONPs) by multiple gradual concentrations and re-tested for biofilm-production by the same method. Results: EDTA and Kojic acid reduced the biofilm-formation ability of the tested isolates by 85.4 % and 65.5% respectively with reduced mean optical density (OD) reading to (0.0267± 0.051) and (0.063± 0.108) respectively with a high statistically significant difference (p < /em><0.0001). A concentration-dependent biofilm-inhibition effect was observed with different concentrations of CuO NPs(125-2000 µg/ml) for all the tested isolates regardless the genus and the species. Conclusion: there was a remarkable biofilm-inhibition effect for the three tested non-antibiotic substances; the use of these substances can provide a solution for the problem of difficulty in treatment of nosocomial infections due to MDR biofilm-forming Gram-negative superbugs.

DOI

10.21608/mid.2022.163207.1404

Keywords

Key words: EDTA, kojic acid, MDR, Biofilm. CuoNPs

Authors

First Name

asmaa

Last Name

thabet

MiddleName

-

Affiliation

Microbiology and Immunology department, Faculty of Medicine, Sohag Unuversity, Egypt

Email

asmaanasreldin81@gmail.com

City

sohag

Orcid

-

First Name

Amira

Last Name

Esmail

MiddleName

-

Affiliation

Microbiology and Immunology department, Faculty of Medicine, Sohag Unuversity, Egypt

Email

amiraesmail92@yahoo.com

City

-

Orcid

-

First Name

Mamdouh

Last Name

Esmat

MiddleName

-

Affiliation

Microbiology and Immunology department, Faculty of Medicine, Sohag Unuversity, Egypt

Email

mmesmat2000@yahoo.com

City

-

Orcid

-

Volume

4

Article Issue

1

Related Issue

39092

Issue Date

2023-02-01

Receive Date

2022-10-22

Publish Date

2023-02-01

Page Start

108

Page End

126

Print ISSN

2682-4132

Online ISSN

2682-4140

Link

https://mid.journals.ekb.eg/article_269413.html

Detail API

https://mid.journals.ekb.eg/service?article_code=269413

Order

16

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Anti-biofilm Effect of Copper Oxide Nanoparticles, EDTA, and Kojic Acid on MDR-biofilm Forming Gram Negative Superbugs in Sohag University Hospitals, Egypt

Details

Type

Article

Created At

25 Dec 2024