Background: Needle stick injuries (NSI) are wounds caused by sharps such as hypodermic needles, blood collection needles, needles used to connect parts of iv delivery systems. It is the most efficient method of transmitting blood-borne infections between patients and healthcare staff, and hepatitis B, hepatitis C and human immunodeficiency virus (HIV).Aim: The study aimed to evaluate the prevalence of sharp injuries infection by HIV and HBV among Sudanese nurses in Khartoum state.Methods: This was a cross sectional study carried out in Ibrahim Malik Hospital- Khartoum State conducted to evaluate the prevalence of sharp needle injuries infection by HIV and HBV in nurses. Sixty nurses working in variant hospitals and centers were enrolled in this study after taking their verbal consent.Results: Out of 60 nurses, 6 (10.0%) have a history of blood transfusion and 54 (90.0%) don't have a history of blood transfusion, 52 (87.0%) were vaccinated against HBV, and 8 (13.0%) were not vaccinated against HBV, 6 (10.0%) have a history of surgical transplantation and 54 (90.0%) don't have a history of surgical transplantation.Out of 60 nurses, 0.0 (0.0%) with positive HIV and HBV, 60 (100%) with negative HIV, HCV and HBV.The results showed negative results of HIV, HCV and HBV among Sudanese nurses in Khartoum state. Conclusion: The study observed that nurses have negative results of HIV, HCV and HBV.