There have been several studies on barriers to physical activity (PA) for university students published in other countries and Saudi Arabia. However, there are no data on individuals associated with Umm Al-Qura University. A total of 238 university students, aged between 18 and 27 years old, participated in this study, which was designed to assess the perceived barriers to PA among Umm Al-Qura University students. The results of this study showed that “lack of accessible places" was the most important barrier to PA. Then, “I don't know about sport facilities" was the second most important. The least important was “I don't have motivation" followed by “I feel lazy". The findings of this study confirmed that university students at Umm al-Qura University may posing highly sedentary lifestyle and physical inactivity, although there is both a sports hall and a gym at the university. Thus, educating students about the place and times is warranted.