In this study, we present a novel approach to enhance power generation in MFCs by incorporating several innovative modifications. The MFC design eliminated the need for a membrane by depositing a hydrophobic PVDF nanofibers layer to facilitate oxygen penetration while preventing water passing. Additionally, a new anode material was developed by sintering corn cob under a nitrogen atmosphere, providing improved performance and cost-effectiveness. The MFC operated as an air-cathode system, eliminating the need for expensive electron acceptors. Furthermore, the MFC was driven by sewage wastewater, demonstrating the potential for waste-to-energy conversion. The power generation performance of the MFC was evaluated and compared to traditional carbon-based anode materials. The results showed a significant increase in power density with the corn cob anode, reaching 515 W/m2. In comparison, the power densities obtained with carbon felt, carbon cloth, and carbon paper anodes were 94, 95, and 140 W/m2, respectively. These findings highlight the superiority of the corn cob anode in terms of power generation efficiency. The demonstrated increase in power density with the corn cob anode highlights the potential for scaling up MFCs for practical applications, including wastewater treatment and remote power sources.