Great interest has been given in the last few years for issues related to environmental protection and safety. This included the use of safe methods for wastewater treatment. One of these methods is the use of natural materials instead of chemicals. Mushroom is used in the present study as a natural flocculent with different dosages for treating synthetically prepared suspension of different concentrations of local clay which is collected from Egypt's River sides. The parameters studied are: pH, mushroom dose, clay dose, settling time and turbidity are determined. Results showed that a maximum turbidity removal of 96.5% is obtained at pH of 6.7 and optimum dose of 0.4 g/L of mushroom and 4000 TSS. By using 0.4 g/l of mushroom, about 17.3 NTU of turbidity remained from an initial turbidity of 100 NTU, while 4.28% of turbidity remains for 400 NTU of initial turbidity. This occurs after 120 minutes. A high turbidity removal of 96.5% with 22 NTU residual turbidity for 320 NTU of initial turbidity, while the lowest is 82.2% for an initial turbidity of 101 NTU.