Agri Coll is a bio-organic fertilizer which manufactured in Soil and Water department, Faculty of Agriculture, Assiut University. A field experiment was conducted in two consecutive seasons (2020/2021 and 2021/2022) to study and evaluate the effect of Agri coll on wheat (Triticum aestivum L.) yield and its components. It was added with irrigation water (fertigation). Treatments include two levels of Agri coll (50 and 100 L/ fed) and recommended dose of mineral fertilizer (nitrogen 100 kg N/ fed, phosphorus 100 kg/fed and potassium level 48 kg/ K₂O) as a control treatment, as well as their combinations. The experiment was designed as a completely random block with four replicates. Results showed that using of Agri coll with irrigation water recorded increases in the yield of wheat straw, and grains, improving yield and its components and concentration of some nutrients. Grain yield increased by 7.33 and 15.38% in the first season and 9.09 and 15.77 % in the second season by addition of 50 and 100 liters / Agir coll respectively. Agri coll applications increased soil nutrients available form and wheat nutrients content compared with control treatment. The obtained results suggest that the manufactured has high potential as a good source of minerals and organic matter and can be substituted with the mineral fertilizers required for optimal crop yield, growth parameters and concentrations of essential nutrients in wheat (Triticum aestivum L.).