One of the zoonotic health problem affecting is cryptosporidiosis. There is currently no 
approved treatment for cryptosporidiosis, apart from nitazoxanide (NTZ). This study evaluated 
the anti-protozoan and immune-modulating properties role of spirulina and probiotics, either 
alone or loaded on Cu-MOF, against Cryptosporidium in immunocompromised mice compared 
to NTZ. Seventy-eight male laboratory-bred pathogen-free, Swiss-albino mice were divided 
into seven groups each of 12 mice except negative control one with six mice as follows: GI: 
infected-untreated (positive control), GII: infected and Spirulina treated, GIII: infected and 
probiotics treated, GIV: infected and NTZ treated, GV: infected and Spirulina loaded on Cu
BTC MOF treated, GVI: infected and probiotics loaded on Cu-BTC MOF treated, GVII: 
neither  infected nor treated (negative control). Drug efficacy was parasitological, biochemical 
and histopathological evaluated.  
The results showed that all drugs significantly reduced oocysts' count and intestinal parasite 
burden as well as improved pathological squeals compared to corresponding infected mice. 
There was a decrease in amyloid and neopterin sera levels in treated mice compared to infected 
ones. NTZ gave the best treated results, followed by Spirulina nano formulation.