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351356

GENISTEIN FOR EXPERIMENTAL MURINE TRICHINELLOSIS: A NOVEL THERAPEUTIC INSIGHT WITH EVALUATION OF IMMUNOLOGICAL AND IMMUNOHISTOCHEMICAL PARAMETERS

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Last updated: 25 Dec 2024

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Abstract

Herbal remedies have been studied as alternative or adjuvant treatment options against parasitic infections.
Treating trichinellosis with albendazole (ABZ) showed many drawbacks despite its efficacy.
This study assessed the effectiveness of genistein (Soybean extract), either with or without ABZ, in
treating experimental murine trichinellosis and evaluated the regulatory mechanisms. One hundred
Swiss albino laboratory-bred mice were categorized into five groups: G1 uninfected and untreated
mice, G2 infected untreated mice, G3 infected and ABZ treated, G4; infected and genistein treated, G5
infected and ABZ &genistein treated. Each group was divided according to the scarification time (7-
or 40-days post-infection (dpi), respectively). The treatment efficacy was evaluated parasitological,
histopathological, immunological, immunohistochemical (GATA3, glutathione peroxidase 1 (GPX1),
and caspase 3) methods and by SEM. Combination of genistein & ABZ in treating trichinellosis showed
the best drug efficacy, with reduction of adults and larvae count at 96.73% & 81.56 %, respectively.
They showed the highest degree in amelioration of histopathological changes and reduction of inflammation
parallel to genistein-treated ones with significant reduction of GATA3 & caspase 3 expressions,
but significant elevation of GPX1 expression as well as highest degeneration of adults and larvae
by SEM.

DOI

10.21608/jesp.2024.351356

Keywords

albendazole, Caspase 3, GATA3, Glutathione peroxidase 1 (GPX1), Genistein (Soybean extract), Immunopathology, Trichinellosis

Authors

First Name

ASMAA

Last Name

IBRAHIM

MiddleName

F.

Affiliation

Department of Clinical & Molecular Parasitology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

Email

dr_asmaafahmy@yahoo.com

City

-

Orcid

0000-0002-1180-1845

First Name

SAHAR

Last Name

SELIM

MiddleName

M.

Affiliation

Department of Clinical & Molecular Parasitology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

Email

-

City

-

Orcid

-

First Name

DALIA

Last Name

SHAFEY

MiddleName

A.

Affiliation

Department of Clinical & Molecular Parasitology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

Email

-

City

-

Orcid

-

First Name

DINA

Last Name

SWEED

MiddleName

M.

Affiliation

Department of Pathology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

Email

-

City

-

Orcid

-

First Name

SHAIMAA

Last Name

FARAG

MiddleName

A.

Affiliation

Department of Clinical & Molecular Parasitology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

Email

-

City

-

Orcid

-

First Name

MARWA

Last Name

GOUDA

MiddleName

A.

Affiliation

Department of Clinical & Molecular Parasitology, National Liver Institute, Menoufia University, Shebin El-Kom, Menoufia Governorate, Postal code: 32511 Egypt

Email

marwa_goda@liver.menofia.edu.eg

City

-

Orcid

0000-0003-1723-6792

Volume

54

Article Issue

1

Related Issue

47264

Issue Date

2024-04-01

Receive Date

2024-04-23

Publish Date

2024-04-01

Page Start

53

Page End

64

Print ISSN

1110-0583

Online ISSN

2090-2549

Article File

JESP_Volume 54_Issue 1_Pages 53-64.pdf

PDF . 1.7MB

Link

https://jesp.journals.ekb.eg/article_351356.html

Detail API

https://jesp.journals.ekb.eg/service?article_code=351356

Order

8

Type

Original Article

Type Code

1,127

Publication Type

Journal

Publication Title

Journal of the Egyptian Society of Parasitology

Publication Link

https://jesp.journals.ekb.eg/

MainTitle

GENISTEIN FOR EXPERIMENTAL MURINE TRICHINELLOSIS: A NOVEL THERAPEUTIC INSIGHT WITH EVALUATION OF IMMUNOLOGICAL AND IMMUNOHISTOCHEMICAL PARAMETERS

Details

Type

Article

Created At

25 Dec 2024