This study was conducted to evaluate the modulatory effect of basil seed powder (BSP) on MSG-induced oxidative stress in rats. In this regard, thirty rats weighing 150 ± 10.86 g were used for the study and separated into 5 groups . Group 1(-): control negative group; group 2(+): MSG only as control positive group; groups 3, 4, and 5 were given MSG and treated with (2.5, 5, and 7.5 g/100 g diet) BSP, respectively. The MSG groups were given a 4 mg/kg b.wt. daily oral dose of MSG. After completing 28 days, the biological parameters were ameliorated by BSP intervention (2.5, 5, and 7.5 g/100 g diet). The use of BSP reversed the negative effects of MSG on liver oxidative stress parameters. The BSP-treated (7.5 g/100 g diet) group recorded an improvement, reaching the point of no significant difference with the negative group in SOD. In addition, our results revealed that the MSG-treated group shows a significant change in deterioration in hematological parameters, liver enzymes, some kidney function, serum glucose, and lipid profile. Interestingly, BSP (2.5, 5, and 7.5 g/100 g diet) administration demonstrated the ability to significantly (p≤0.05) alleviate these toxic effects dose-dependently. Histological observations of liver tissue provided evidence for the ameliorative effect of BSP. The study noted the harmful effects of MSG and suggested BSP as a potential remedy for MSG oxidative stress