The current research aimed to investigate the prevalence of antibiotic resistance among Staphylococcus aureus and Escherichia coli. About 72 samples were suspected to have urinary tract infections and were further identified by using a biochemical and VITIK 2 compact system. The results showed that 21 samples were diagnosed as Staphylococcus aureus isolates and 25 samples were diagnosed as Escherichia coli isolates. The VITIC 2 compact system was applied to determine the bacterial isolates' sensitivity to antibiotics. The results showed that E. coli isolates were 100% resistant to Cephalexin, Amoxicillin, Penicillin, and Amoxicillin-clavulanic acid, while S. aureus showed 100% resistance against Cefepime and Cefixime. In another hand, the lower resistance of E. coli showed against Clindamycin and Erythromycin was 0.0%, besides the same resistance ratio of S. aureus against Imipenem, Nalidixic acid, Trimethoprim / Sulfamethoxazole and Cefotaxime.