X-ray analysis was applied to estimate the percentages of heavy metals in ovarian tissues of the beetle Calosoma olivieri. This research was performed to assess the influence of heavy metals on biochemicals in ovarian tissues in the ground beetle Calosoma olivieri. A total of 320 beetles with an average body weight of 1.96 g were obtained from two locations the garden of the Faculty of Science, Alexandria University, Alexandria, Egypt, which was previously classified as an uncontaminated zone, and a site surrounding the KZ factory of pesticides and chemicals. The results revealed a high activity level of MDA, Low activity level of glutathione-S – transferase GST, Glutathione Peroxidase- GPx, Reduced glutathione GSH and glutathione reductase GR, Ascorbate-Peroxidase APOX, Superoxide dismutase SOD, Catalase activity CAT, on the other side high concentration of Protein carbonyl. Our data showed the detection of heavy metals in soil samples collected from the polluted group more than those of control site samples. The results showed that C. olivieri is a valuable indicator of heavy metal pollution. Moreover, the metrics under examination are valuable markers for evaluating the deleterious consequences of environmental pollutants.