The River Nile represents the main source of water in Egypt, used for irrigation, drinking, and fisheries. Therefore, it is so necessary to undergo frequent monitoring of the protozoan biodiversity in fresh water to avoid epidemic diseases. Accordingly, this work aims to isolate and identify the expected protozoan species in water samples collected from the River Nile and taps at four sites in Dakahlia Governorate. Water samples were collected using the concentration technique, followed by modified Ziel-Neelsen stain and Giemsa staining methods for detecting protozoan species using bright light and phase-contrast microscopy. 12 protozoan species belonging to the subphylum Sarcodina, Masigophora, and Cilliophora were recorded. Moreover, examination of water samples revealed that drinking water was contaminated by six species of protozoa. A description of the locomotive and cyst stages is carried out for most of the detected protozoa. Conclusion: This study proved that drinking water must be carefully analyzed to avoid the presence or growth of protozoa