Staphylococcus aureus has been subjected to low-level laser radiation at various exposure periods. From January 2019 to September 2019, 140 skin and UTI  samples (swabs) were obtained from patients at Medical City and Medical City Teaching Hospital who had burns, wounds, and UTI s infections. 50 isolates of S. aureus were identified through cultural traits, microscopic examination, biochemical tests, API staph, and the Vitek system. The 15 isolates were subjected to a diode laser at an exposure length of 1 minute, 3 minutes, and 5 minutes. Before and after irradiation, it was evaluated how active the bacteria were in producing alpha toxin and how sensitive they were to antibiotics. The results of the alpha toxin generation of 15 irradiated isolates showed that the amount of toxin produced decreased at different irradiation exposure times [1, 3, and 5 min.]. The diode laser's impact on S. aureus' sensitivity to antibiotics results in a small increase in the diameter of the drugs' inhibition zone at various exposure times.