Multidrug-resistance (MDR) is a major and an on-going public health problem globally. It occurs mainly among Gram-negative pathogens of Enterobacteriaceae such as Klebsiella pneumoniae, Pseudomonas aeruginosa and recently observed in Acinetobacter baumannii, Acinetobacter baumannii are Gram-negative opportunistic bacteria with low virulence properties. Their resistance to antibiotics has become a problem of concern in hospital infections and one of the pathogens responsible for healthcare associated infections (nosocomial). A total of 117 clinical samples of Acinetobacter spp. were collected through anonymous sources from the clinical microbiology laboratory and compartment or inanimate objects, and they were identified phenotypically 78 isolates were detected phenotypically as A.baumannii and 47/78 isolates (59.7%) were detected as MDR A.baumannii. This type of resistance are already widespread in certain parts of the world, particularly Europe, Asia and South America, while the situation in other places such as Africa is not well documented. This study aimed to investigate the multi-drug resistance profile of A.baumannii from different infection sites, and it's spreading in Egypt.