The present in vitro study aimed to investigate the ability of propolis and pollen as honey bee products in treatment of tumor. As well as, using them and their water extract as in-functional food which have pharmacological properties and antioxidant compounds. This i study was performed on tumor cells (caco2 cell and hepG2 cell) in vitro. The chemical composition, Total phenols, Total flavonoids and antioxidant activity of DPPH were estimated. Fractionation and identification of phenolic compounds and flavonoids compounds were determined by HPLC. Also, the water extracts of propolis and pollen were used in crackers product. The results indicated that the raw propolis and pollen were higher than their water extracts for total phenols and total flavonoids. While, water extract of propolis has higher antioxidant activity by DPPH than raw samples. Moreover, raw propolis and pollen have more phenolic, flavonoid compounds contents than their water extract by HPLC. Also, raw pollen has the highest level of caffeine, oleuropin, ferulic and ellagic (36.51, 16.07, 28.41, and 18.65 mg/100g, respectively) as a phenolic compounds. Antitumor assay indicated that water extract of propolis has (IC₅₀ equal to 123.55) more than water extract of pollen (IC₅₀is 113.61) for colon cancer (caco₂). While, water extract of pollen has (IC₅₀equal to 825.25) more than water extract of propolis (IC₅₀ is 352.2) for liver cancer (HepG₂). The crackers samples which contained a high percent (1, 5%) of water extract of propolis or pollen has the highest scores of taste, flavor, texture and general appearance. In conclusion the results of the current study indicated that bee propolis can be used as anticancer for colon cancer and bee pollen as anticancer for liver cancer. As well as, propolis and pollen can be used in functional food.