Natural products are used to develop anti-ulcer drugs with minimal side effects. In the present study pomegranate juice samples fortified with peel powder or seed homogenate were investigated for the treatment of induced peptic ulcer in rats. Forty-two female Albino rats weighing about 150±5 g were divided into two groups. The first group was fed only the basal diet as a negative. The second major group was randomly divided into six groups (six animals each). Group (2) the Positive control group (ve+) received orally (2 ml/kg BW) distilled water/day/rat by epi-gastric tube. Group (2) pretreated orally with 5mg/day (P. juice). Group (3) pretreated orally with (5mg/day) from (1.5 g PPP /100 ml P. Juice). Group (4) pretreated orally with (5mg/day) from (1.5 g PSP /100 ml P. Juice). Group (5) was pretreated orally with (a 5g/ 100 g diet /day) from (PPP). Group (6) received orally (50 mg/kg BW) Antodine® suspended in distilled water. After 7 days, animals were fasted for 24 hrs. Induction of ulcers in The second major group was done on the last day of the experiment.to induce acute gastric mucosal lesion, ibuprofen was treated orally in a dose of 200 mg/kg body weight three times a day at an interval of 8 hrs. Ulcer index, protective index, volume of gastric juice, and pH of gastric juice were evaluated in all groups. In biochemical parameters, Malondialdehyde (MDA), Reduced glutathione (GSH) concentration, Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, Total protein, Albumin, and Alkaline phosphatase (ALP) activity were determined. The results indicated that the macroscopic and lipid peroxidation in the stomach had significant anti-ulcer activity of pomegranate juice, pomegranate juice enriched with peel powder and seed homogenate, peel powder, and Antoine. The anti-ulcer activity was comparable to the positive control. The results also indicated that there were no significant differences in values of physicochemical parameters including pH, titratable acidity, and TSS among the control rats group and fortified juice sample groups, especially at the level of (1.5 g/100 mL) either in peel powder or seed homogenate. The fortification treatments caused significant differences in the total phenolic, antioxidant activity and total flavonoid content among studied samples. Nineteen phenolic compounds had identified with significant variation among the studied samples. Seventeen volatile compounds were detected in the aroma profiles of pomegranate juice and selected supplemented pomegranate juice samples. Fortified pomegranate juice with peel powder recorded an increase of fenchone and a-terpineol to 5.39% and 4.21%, respectively compared to fresh pomegranate juice. The pretreatments with pomegranate and its derivatives did not produce significant changes in the tested biochemical parameters and histopathology in Female rats with Ibuprofen-induced gastric ulcer. It was concluded the  fortification of pomegranate juice by peel powder or seed homogenate could be considered a promising gastroprotective and anti-ulcerative agent.