Background : Endophytic fungi have a wealth of bioactive compounds that are specific to their plant hosts. These compounds benefit a variety of purposes and include antioxidants, immunosuppressant. Aim of study: Investigation to examine the bioactive component of the dichloromethane extract of three endophytic fungi that were isolated from three distinct host plants. The current study used Gas Chromatography-Mass Spectrometry (GC-MS) to analyze the crude extract from secondary metabolites produced from endophytic fungi. Methods: The endophytic fungi Aspergillus fumigatus, Alternaria tenuissima, and Nigrospora zimmermanii were isolated from twigs and leaves of Tamarix aphylla, Trigonella foenum-graecum, and Nerium oleander, respectively. The isolated fungi were identified phenotypically and molecularly using universal and specific primers. The fungi were grown in a liquid fermentation medium Potato Dextrose Broth at 25°C for 30 days. The organic solvent dichloromethane was used in this test, and these extracts were analyzed by Thin Layer Chromatography (TLC) Gas Chromatography-Mass Spectrometry (GCMS). Results: The results showed presence of many Active chemical compounds in the crude extracts produced from the three tested fungi and identified by (TLC) and (GC-MS) analysis. Conclusion : The findings of this study indicate that a broad range of endophytic fungi are capable of producing bioactive compounds that can be used to cure a number of diseases.