Cedrus libani and Pinus brutia are Pinaceae family members that are used in traditional medicine for rheumatism. This study aimed to investigate the anti-inflammatory and analgesic activity of ethanolic extract fractions of both plants and determine the main phytochemicals responsible for these activities. The anti-inflammatory activity was assessed in vitro using albumin denaturation assay and in vivo with carrageenan-induced edema in rats. The analgesic effect was assessed in vivo using the formalin test and the tail flick test, by using sodium diclofenac as a reference drug. Results showed that the aqueous fraction (AQ) of cedar extract and the ethyl acetate fraction (EA) of pine extract suppressed albumin denaturation more than the other fractions (p<0.05). The AQ fraction, either intraperitoneally (30mg/kg) or topically (1% gel), could also significantly reduce edema caused by carrageenan better than the EA fraction, and both fractions have more activity than sodium diclofenac (p<0.001). Furthermore, the EA fraction decreased the animals' nociceptive response in both phases of the formalin test and outperformed sodium diclofenac. Whereas, the AQ fraction exerted the same activity as diclofenac (p>0.05). AQ and EA fractions also demonstrated analgesic effects by increasing pain latency in the tail flick test in a way that was better or comparable to diclofenac. These effects may be related to polyphenols in fractions, including flavonoids and tannins. In conclusion, the AQ and EA fractions possessed significant anti-inflammatory and analgesic activity compared to sodium diclofenac, making them a novel therapeutic drug.