Now a days the biomaterials made of sericin from waste Bombyx mori cocoon has created elevated interest among research community due to their high biocompatibility with wide range of cell lines. In our present study, we reported the synthesis of cerium oxide nanoparticles (CeONPs) from the plant extract of Securinega leucopyrus and incorporated in sericin hydrogel for the development of novel nanocomposite for wound healing applications. The plant extract acts as both reducing as well as stabilizing agent which helps in the conversion of cerium nitrate to cerium oxide. To analyze the properties of the synthesized nanoparticles various physiochemical characterizations such as UV-visible spectrophotometry, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) were performed. UV-Visible spectrum of the synthesized nanoparticle showed distinct peak at 240 nm. The size and charge of the nanoparticle was investigated using DLS and was found to be 186.7 nm and -22.5 mV. The plant extract and the cerium oxide nanoparticles also showed antibacterial activities against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) microorganisms. The plant extract and the nanoparticles synthesized showed anti-oxidant activity with radical scavenging of 83.4 % and 76.48 % respectively. The hydrogel also exhibited good water retention property and swelling behavior. The drug release kinetics were studied for obtained sericin hydrogel. The plant extract and CeONPs loaded hydrogels also exhibited good antibacterial activity. Hence, our results conclude that sericin nanocomposite hydrogel could be the potential candidate in healing chronic wounds.