An intrauterine device (IUD) is one of the most often used contraceptive tools. However, it represents a stable surface for microbial attachment and a perfect environment for the biofilm to grow and thrive. Thus, it might act as an infection reservoir of the reproductive system. This study aimed to isolate and identify microbes forming biofilms on IUDs and to provide some remedial measures to counteract biofilm formation. A total of 110 IUD samples were collected from women attending family planning clinics. They were treated to isolate and identify microorganisms forming biofilms by conventional methods. Biofilm assay was done using the tissue culture plate method to assess the isolates' degrees of biofilm formation and test the effect of selected antibiotics and antibiofilm agents on biofilm formation and disruption of preformed biofilm. A scanning electron microscope was also used to assess the effect of the tested agents on biofilm formation and disruption of preformed biofilms on IUD segments. 177 isolates were recovered from 110 IUDs including Candida spp. (51, 28.8%), Staphylococcus aureus (49, 27.7%), coagulase-negative Staphylococci (28, 15.8%), Pseudomonas spp. (21, 11.9%), E. coli (16, 9%) and Klebsiella spp. (12, 6.8%). Klebsiella spp. was the most biofilm producer, different isolates showed variable degrees of biofilm formation. The tested antibiotics exhibited remarkable inhibitory effects of biofilm formation either alone or in combination with Chitosan and N-acetyl cysteine as antibiofilm agents.