Materials & Methods: A total of seventy two male wistar rats were included in the study. Early lung cancer was induced by urethane in the rats except for the control group. Rats were divided randomly into 7 groups (10 animals each) as follows: I-Control, II-cancer, untreated, III-cancer + free cisplatin alone IV- cancer + cisplatin nanoparticles alone V- cancer + free cisplatin + free curcumin VI-cancer + cisplatin nanoparticles + curcumin nanoparticles, VII- cancer + cisplatin nanoparticles + free celecoxcib.

Results: Treatment of lung cancer rats with either free or nanoparticles of cisplatin alone showed a significant suppression of the plasma levels of the CYFRA21-1 tumor marker, as well as the tissue expressions of the pro-inflammatory, proliferative & antiapoptotic parameters as compared to those of group II cancer rats. Moreover , the free form of curcumin or its nanoparticles or free celecoxcib adjuvants supplementation combined with cisplatin nanoplarticles led to a further decrease of all parameters as compared to those treated with cisplatin alone, that was more evident in the group treated with cisplatin nanoparticles combined with curcumin nanoparticles adjuvant.

Conclusion: The results of the present study emphasized the synergestic effect of cisplatin nanoparticles combined with either forms of curcumin or free celecoxib adjuvants as promising efficient new nanotherapy against lung cancer. To the best of our knowledge, it is the first study that established the urethane induced experimental lung cancer model in Upper Egypt, along with its treatment trial with cisplatin & curcumin nanoparticles adjuvant therapy.