The most common pain among hemodialysis patients is caused by the cannulation of arteriovenous fistula due to the diameter and length of these needles. This study aimed to compare the impact of lidocaine spray and ice spray on the intensity of pain during the IV insertion in hemodialysis patients and examine the amount of pain relief during this intervention. This is a triple-blind clinical trial that was conducted on 50 patients between 18 to 65 years of age who needed the insertion hemodialysis catheters. Each patient received a total of three sprays, one type of spray per session. The insertion pain was measured using the VAS scale. It took 10 minutes from applying the lidocaine spray to disinfection and injection. However, the process of disinfection and insertion started immediately after applying ice spray and control spray (70% alcohol). Wilcoxon and Friedman tests (non-parametric) were carried out to examine the results of the research, and SPSS V16 was used to analyze the data. According to the mean values, there is a significant difference in the pain scores of ice spray, lidocaine spray, and control spray (alcohol), indicating that lidocaine spray is more effective than ice and alcohol sprays in reducing the intensity of the pain caused by dialysis needles. (P=0.010). Compared with ice spray, lidocaine spray results in a greater reduction in the pain caused by hemodialysis cannulation. Therefore, this method can be used as a proper, inexpensive, accessible, and easy approach to reducing the pain of inserting hemodialysis vascular needles.