Commiphora myrrha has been extensively used in traditional medicine. The biochemical characters of Commiphora myrrha hydro-methanolic and petroleum ether extracts were studied. The phenolic and flavonoid contents, DPPH assay, in vitro anti-inflammation activity of them by determining the membrane stabilizing activity and NO assay as well as chemical profile by GC-MS and HPLC were investigated. The results showed that phenolic and flavonoid content of hydro-methanolic extract (158.26 ± 3.44 mg Gallic acid equivalent (GAE)/g of extract and 91.43 ± 3.33 Rutin /g extract, respectively was higher than those of petroleum ether (126.16 ± 0.42 mg GAE/g of extract and 61.97 ± 2.5 mg Rutin /g extract, respectively). Also, hydro-methanolic has potent antioxidant activity than petroleum ether (IC50 = 46.56 ± 1.06 and 66.13 ± 1.64 μg/mL, respectively). Moreover, hydro-methanolic extract exhibited strong stabilizing effects on human red blood cell membrane as well as strong NO production inhibition. HPLC for hydro-methanolic extract showed 13 phenolic compounds and 8 flavonoid one. While, GC-MS analysis exhibited 49 compounds in the petroleum ether extract and 45 ones in the hydro-hydro-methanolicic extract. In general, the anti-inflammatory effect is correlated to antioxidant activity of the plant depends on the phenolic and flavonoid content.