The objective of this research was to identify the primary constituents of the essential oil obtained from Cotula cinerea (Delile) Vis. through the process of hydrodistillation. Additionally, the study aimed to explore the oil's in vivo wound healing properties and its in vitro antioxidant capabilities. Several compounds were identified in the essential oil, including thujone, santolina triene, camphor, and 1,8-cineole. To assess its wound healing potential, the essential oil was topically applied to induced wounds in mice, resulting in a notable acceleration of wound healing and repair, with a reported rate of 93.2 ± 0.7%. Furthermore, the research included an evaluation of the essential oil's analgesic activity, which was assessed by administering intraperitoneal injections of acetic acid to mice. Moreover, the antioxidant activity of the essential oil was assessed through the ferric reduction antioxidant power and DPPH scavenging assay. The findings from these antioxidant tests revealed that the essential oil extracted from Cotula cinerea exhibited the ability to reduce iron and scavenge DPPH radicals effectively. These results underscore the bioactive characteristics of Cotula cinerea essential oil and its potential for synergistic effects. Consequently, Cotula cinerea shows promise as a natural source of biologically active compounds with therapeutic potential for wound healing. These discoveries suggest that Cotula cinerea could serve as a viable alternative to synthetic wound treatment medications in the future pharmaceutical industry.