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327685

THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS

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Last updated: 04 Jan 2025

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Abstract

 
Impaired healing process of diabetic wounds is a common problem among diabetic patients. These diabetic wounds are slow to heal and can lead to serious complications if left untreated. One of the reasons diabetic wounds are slow to heal is that pathogenic bacteria often colonize them. Cold atmospheric plasma (CAP) is a promising new technology that has been shown to effectively kill and inhibit pathogenic bacteria in diabetic wounds. In the current study, we examined by what means CAP works and its potential impact as a diabetic wound treatment. Fifty diabetic patients aged from 20 to 60 years old with diabetic wounds were included in our study using the swab method. We used general and specific culture media to isolate the pathogenic bacteria from diabetic wounds and identified the bacteria via different techniques using a light microscope (oil lens) and VITEK. Moreover, we confirmed the identification of isolated bacteria by 16S rRNA. Our results demonstrated the direct anti-bacterial effect of CAP against the isolated bacteria. The most prominent bacterial type in diabetic wounds was Staphylococcus sp. CAP demonstrated the highest effect against Bacillus sp. and Paenibacillus sp., which were responsible for relevant changes in the skin and were associated with the skin immune response and microbiota. Our results revealed the therapeutic use of CAP as a powerful strategy to overcome the invading pathogenic bacteria that colonize diabetic wounds and hence improve the healing process of diabetic wounds.

DOI

10.21608/bfsa.2023.327685

Keywords

bacterial colonization, Cold atmospheric plasma, Diabetic wounds, Paenibacillus species, pathogenic bacteria

Authors

First Name

Gamal

Last Name

Badr

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Assiut University

Email

gamal.badr@aun.edu.eg

City

Assiut

Orcid

0000-0002-6157-7319

First Name

Fayez

Last Name

El-Hossary

MiddleName

M.

Affiliation

Physics Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt

Email

fayez1004@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Salah

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt

Email

msalah61082@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Khalaf

MiddleName

-

Affiliation

Physics Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt

Email

mmmfoas@gmail.com

City

-

Orcid

0000-0003-0529-6720

First Name

Eman

Last Name

Sayed

MiddleName

A.

Affiliation

Zoology Department, Faculty of Science, Assiut University, 71516 Assiut, Egypt

Email

emanabdo603@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Elminshawy

MiddleName

-

Affiliation

Department of Cardiothoracic Surgery, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

aelminshawy@aun.edu.eg

City

-

Orcid

-

Volume

46

Article Issue

2

Related Issue

44483

Issue Date

2023-12-01

Receive Date

2023-06-08

Publish Date

2023-12-01

Page Start

1,285

Page End

1,300

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 46_Issue 2_Pages 1285-1300.pdf

PDF . 1.6MB

Link

https://bpsa.journals.ekb.eg/article_327685.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=327685

Order

327,685

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

THE THERAPEUTIC POTENTIAL OF COLD ATMOSPHERIC PLASMA AGAINST PATHOGENIC BACTERIA INHABITING DIABETIC WOUNDS

Details

Type

Article

Created At

25 Dec 2024