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327651

THE EFFECT OF ESSENTIAL OILS OF SELECTED PLANTS ON CLINICAL ISOLATES OF CANDIDA SPECIES GROWTH, TRANSITION AND BIOFILM FORMATION

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Last updated: 04 Jan 2025

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Abstract

Candida species are one of common opportunistic pathogens in humans. Detection of antifungal resistance between candida species become increasing, a great  interest in creating new antifungals utilizing natural sources like plant essential oils (EOs) . This study aimed to evaluate the effect of EOs of selected plants on Candida species growth, transition, and biofilm formation as well on the expression levels of certain virulence genes. Patients and methods: Fifty candida isolates were collected from napkin dermatitis and oral candidiasis patients, different Candida species were identified phenotypically. Essential oils for some medicinal plants (basil, clove, garlic, and peppermint) were extracted by hydro-distillation. Their antifungal activity was assessed by minimal inhibitory concentration methods. In addition the effect of EOs on different Candida ultrastructure was detected by scanning electron microscope, the antibiofilm characteristics were examined by microtiter plate method. The ability of tested EOs to interfere with transition of candida from blastopore to hyphae form was evaluated. The effect of basil and clove oils on various virulent genes was determined by analyzing the expression levels of HWP, ALS3, SAP3 genes by RT-PCR. Results: C. albicans was the most prevalent species. For all tested Candida isolates, the EOs displayed a high antifungal activity. The EOs decreased the Candida transition, disrupted the Candida ultrastructure and suppressed   their ability of biofilm formation, clove oil exposure significantly downregulated the expression of HWP1, ALS3 and SAP3 genes, in addition basil oil downregulated the expression of ALS3 and SAP3.Conclusion:  This study concluded that clove oil is the best essential oil among other EOs that inhibited growth, transition and biofilm formation by lowest MIC and downregulated HWP1, AlS3 and SAP3 genes, which suggest their potential application for its use as antifungal therapy.

DOI

10.21608/bfsa.2023.327651

Keywords

essential oil, C. albicans, Biofilm, HWP1, ALS3, SAP3 genes

Authors

First Name

Alaa

Last Name

Amen

MiddleName

G

Affiliation

Al-Rajhy Hospital, Assiut University, Assiut, Egypt

Email

alaagalal1992@gmail.com

City

-

Orcid

-

First Name

Ehsan

Last Name

Hassan

MiddleName

AB

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Sherein

Last Name

Elgendy

MiddleName

G

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

shereinelgendy@aun.edu.eg

City

-

Orcid

-

First Name

Soad

Last Name

Bayoumi

MiddleName

AL

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt

Email

soad.bayoumi@pharm.aun.edu.eg

City

-

Orcid

-

First Name

Muhamad

Last Name

Abdel Hameed

MiddleName

R

Affiliation

Department of Internal Medicine & Hematology Unit, Assiut University Hospitals Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Abd-Alrahman

MiddleName

A

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine for Girls, Al-Azhar University, Assiut, Egypt

Email

eman.ahmed.19@azhar.edu.eg

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-

Orcid

-

Volume

46

Article Issue

2

Related Issue

44483

Issue Date

2023-12-01

Receive Date

2023-04-30

Publish Date

2023-12-01

Page Start

1,209

Page End

1,232

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 46_Issue 2_Pages 1209-1232.pdf

PDF . 2.3MB

Link

https://bpsa.journals.ekb.eg/article_327651.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=327651

Order

327,651

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

THE EFFECT OF ESSENTIAL OILS OF SELECTED PLANTS ON CLINICAL ISOLATES OF CANDIDA SPECIES GROWTH, TRANSITION AND BIOFILM FORMATION

Details

Type

Article

Created At

25 Dec 2024