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IN VITRO PROPAGATION OF ALGERIAN LAVANDULA STOECHAS AND ASSESSMENT OF BIOCHEMICAL COMPOSITION AND THEIR ANTIOXIDANT ACTIVITY

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Last updated: 25 Dec 2024

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Abstract

The present research aimed to investigate the effect of plant growth regulators (PGRs) on the in vitro proliferation of Lavandula stoechas shoots and assessment of the antioxidant potential and total phenolic content of extracts obtained from in vitro cultures and wild plants of L. stoechas, as well as, the chemical analysis of the phenolic content of both extracts carried out using HPLC-DAD. The optimal shoot proliferation and biomass accumulation were achieved with the combination of 0.5 mg/l 6-benzyladenine (BA) and 0.5 mg/l gibberellic acid (GA3). The hydroalcoholic extract obtained from in vitro cultures contained the highest amount of phenolic compounds (83.18 mg GAE/g DW extract) compared to the total phenolic content of wild plants extract (32.33 mg GAE/g DW extract). Furthermore, the hydroalcoholic extract from in vitro cultures exhibited a higher antiradical effect against 2,2-diphenyl-1-picrylhydrazyl (DPPH) with IC50 value of 51.38 ± 0.7µg/ml and higher antioxidant capacity (757.26 mg GAE/g DW) observed by total antioxidant capacity (TAC) assay. The chemical profile of both extracts revealed the presence of naringenin, acacetin, rhamnetin, luteolin 3'- 7 diglucoside, and hesperetin. Overall, the results suggest that in vitro cultures of L. stoechas could serve as a potential source for producing active metabolites with high antioxidant properties.

DOI

10.21608/bfsa.2023.327719

Keywords

micropropagation, Phenolic compounds, Free radical scavenging, lavender, HPLC-DAD

Authors

First Name

Roukia

Last Name

Benyammi

MiddleName

-

Affiliation

Laboratory of Genetic Resources and Biotechnologies, Ecole Nationale Supérieure Agronomique (ENSA), El Harrach, Algiers, Algeria.

Email

roukia.benyammi@g.ens-kouba.dz

City

Algiers

Orcid

0000-0002-2975-7838

First Name

Soumeya

Last Name

Krimat

MiddleName

-

Affiliation

Laboratory of Bioactive Molecules and Biomass Valorization, Ecole Normale Supérieure de Kouba (ENS Kouba), B.P N°92 16308 Algiers, Algeria Department of Natural Sciences, Ecole Normale Supérieure de Kouba (ENS Kouba), B.P N°92 16308 Algiers, Algeria

Email

krimatsoumia.2012@gmail.com

City

-

Orcid

-

First Name

Malika

Last Name

Alili

MiddleName

-

Affiliation

Department of Natural Sciences, Ecole Normale Supérieure de Kouba (ENS Kouba), B.P N°92 16308 Algiers, Algeria

Email

malika.alili@g.ens-kouba.dz

City

-

Orcid

0000-0002-2976-3875

First Name

Mohamed

Last Name

Bekhouche

MiddleName

-

Affiliation

Laboratory of Genetic Resources and Biotechnologies (LRGB), Ecole Nationale Supérieure Agronomique (ENSA), Avenue Hassan Badi, El Harrach, Algiers, Algeria

Email

mohamed87bekhouche@gmail.com

City

-

Orcid

-

First Name

Oumaima

Last Name

Touari

MiddleName

-

Affiliation

Department of Natural Sciences, Ecole Normale Supérieure de Kouba (ENS Kouba), B.P N°92 16308 Algiers, Algeria

Email

oumaima.taouari@g.ens-kouba.dz

City

-

Orcid

-

First Name

Nawel

Last Name

Belalia

MiddleName

-

Affiliation

Laboratory of Genetic Resources and Biotechnologies (LRGB), Ecole Nationale Supérieure Agronomique (ENSA), Avenue Hassan Badi, El Harrach, Algiers, Algeria

Email

nawel.belalia@yahoo.fr

City

-

Orcid

-

First Name

Amina

Last Name

Missoum

MiddleName

-

Affiliation

Department of Chemistry, Ecole Normale Supérieure de Kouba (ENS Kouba), B.P N°92 16308 Algiers, Algeria

Email

amina.missoum@g.ens-kouba.dz

City

-

Orcid

-

First Name

Lakhdar

Last Name

Khelifi

MiddleName

-

Affiliation

Laboratory of Genetic Resources and Biotechnologies (LRGB), Ecole Nationale Supérieure Agronomique (ENSA), Avenue Hassan Badi, El Harrach, Algiers, Algeria

Email

khelifi.lakhdar@gmail.com

City

-

Orcid

-

First Name

Abdelkader

Last Name

Morsli

MiddleName

-

Affiliation

Laboratory of Genetic Resources and Biotechnologies (LRGB), Ecole Nationale Supérieure Agronomique (ENSA), Avenue Hassan Badi, El Harrach, Algiers, Algeria

Email

morsli.abdelkader69@gmail.com

City

-

Orcid

-

Volume

46

Article Issue

2

Related Issue

44483

Issue Date

2023-12-01

Receive Date

2023-04-22

Publish Date

2023-12-01

Page Start

899

Page End

911

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 46_Issue 2_Pages 899-911.pdf

PDF . 985.0kB

Link

https://bpsa.journals.ekb.eg/article_327719.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=327719

Order

327,719

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

IN VITRO PROPAGATION OF ALGERIAN LAVANDULA STOECHAS AND ASSESSMENT OF BIOCHEMICAL COMPOSITION AND THEIR ANTIOXIDANT ACTIVITY

Details

Type

Article

Created At

25 Dec 2024