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301132

ASSOCIATION BETWEEN ANTIBIOTIC RESISTANCE, BIOFILM FORMATION AND LASB GENE IN PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SPECIMENS

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Last updated: 25 Dec 2024

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Abstract

A microorganism's capacity to form a biofilm is seen as a sign of a clinically significant infection. Pseudomonas aeruginosa infections are challenging to treat since the majority of isolates have high levels of innate resistance to numerous antibiotics and a propensity to develop biofilms. A total of 350 specimens were collected from patients. 125 isolates of P. aeruginosa were recovered from different clinical samples. The antibiotic susceptibility of these isolates against Ciprofloxacin, Amikacin, cefepime, Norfloxacin, imipenem, Genamicin, Tobramycin, Aztreonam, Piperacillin-tazobactam and Colistin was determined using disk diffusion method.  The TCP technique assay was chosen to identify the development of biofilm. Elastase gene detection was carried out using polymerase chain reaction (PCR) (lasB). The antibiotics against which there was the greatest resistance were cefepime (92.8%) and ciprofloxacin (67.2%). TCP technique identified 15 as weak or non-biofilm producers, 32 as moderate, and 78 as robust producers of biofilm. In 89.6% of P. aeruginosa isolates, LasB was found. P. aeruginosa's pattern of antibiotic resistance was more prevalent in biofilm producers than in non-producers. We come to the conclusion that P. aeruginosa biofilm development and drug resistance have a positive association.

DOI

10.21608/bfsa.2023.301132

Keywords

Biofilm, Pseudomonas aeruginosa, TCP, Antibiotic resistance, lasB

Authors

First Name

Shimaa

Last Name

Ghanem

MiddleName

M.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt

Email

shimaa_ganem@mu.edu.eg

City

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Orcid

Shimaa M. Ghanem

First Name

Gamal

Last Name

Gad

MiddleName

F. M.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt

Email

-

City

-

Orcid

-

First Name

Rehab

Last Name

Abd El-Baky

MiddleName

Mahmoud

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt / Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 11566, Egypt

Email

-

City

-

Orcid

-

First Name

Nancy

Last Name

F. M. Waly

MiddleName

G.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt

Email

-

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-

Orcid

-

Volume

46

Article Issue

1

Related Issue

41495

Issue Date

2023-06-01

Receive Date

2022-09-29

Publish Date

2023-06-01

Page Start

421

Page End

431

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 46_Issue 1_Pages 421-431.pdf

PDF . 815.2kB

Link

https://bpsa.journals.ekb.eg/article_301132.html

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https://bpsa.journals.ekb.eg/service?article_code=301132

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301,132

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

ASSOCIATION BETWEEN ANTIBIOTIC RESISTANCE, BIOFILM FORMATION AND LASB GENE IN PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SPECIMENS

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Article

Created At

25 Dec 2024