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300912

DEVELOPMENT OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF NEBIVOLOL HYDROCHLORIDE, VALSARTAN AND HYDROCHLOROTHIAZIDE IN CO-ADMINISTRATED ANTIHYPERTENSIVE AGENTS AND HUMAN P

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Last updated: 25 Dec 2024

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Abstract

Reversed phase high performance liquid chromatography combined with photodiode array (HPLC-PDA) detector method was developed for simultaneous determination of two binary antihypertensive combinations; either Nebivolol (NEB) with Hydrochlorothiazide (HCZ) or with Valsartan (VAL) in pharmaceutical formulation and  spiked human plasma. The method was successfully applied to separate the three drugs on one chromatogram under the same chromatographic conditions that used for two binary combinations. The separation was established on Xterra C18 column using gradient mobile phase which composed of acidified water of pH = 3.0 (adjusted with 0.1% orthophosphoric acid) and acetonitrile with flow rate of 1.0 mL/min. The drugs were determined at 280 nm. Concentration range of each analyte was shown linearity at 5.0–70.0 μg/mL for NEB, 8.0–60.0 μg/mL for HCZ and 5.0-80.0 μg/mL for VAL. The results of method validation were displayed according to ICH guidelines. The green assessment of the proposed has been carried out using two different tools.

DOI

10.21608/bfsa.2023.300912

Keywords

nebivolol, Valsartan, Hydrochlorothiazide, Antihypertensive drugs, RP-HPLC, Green assessment

Authors

First Name

M.

Last Name

Rizk

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Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, P.O. Box 11795, Cairo, Egypt

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City

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Orcid

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First Name

Maha

Last Name

Abou El‐Alamin

MiddleName

M.

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, P.O. Box 11795, Cairo, Egypt

Email

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City

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Orcid

-

First Name

Shereen

Last Name

Mowaka

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, P.O. Box 11795, Cairo, Egypt /Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El-sherouk city 11837, Egypt

Email

shereen.hassib@bue.edu.eg

City

-

Orcid

-

First Name

Mariam

Last Name

Mohamed

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, The British University in Egypt, El-sherouk city 11837, Egypt

Email

mariam.elhaddad17@gmail.com

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-

Orcid

0000-0001-5535-8497

Volume

46

Article Issue

1

Related Issue

41495

Issue Date

2023-06-01

Receive Date

2022-10-08

Publish Date

2023-06-01

Page Start

157

Page End

172

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 46_Issue 1_Pages 157-172.pdf

PDF . 1.4MB

Link

https://bpsa.journals.ekb.eg/article_300912.html

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https://bpsa.journals.ekb.eg/service?article_code=300912

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300,912

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

DEVELOPMENT OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF NEBIVOLOL HYDROCHLORIDE, VALSARTAN AND HYDROCHLOROTHIAZIDE IN CO-ADMINISTRATED ANTIHYPERTENSIVE AGENTS AND HUMAN PLASMA

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Article

Created At

25 Dec 2024