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Biochemical and Molecular Identification of the Most Common Streptococci Affecting Common pandora (Pagellus erythrinus Linnaeus, 1758) From the Mediterranean Coast of Tripoli

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Fish and aquatic medicine

Abstract

Streptococcosis is one of the septicaemic bacterial diseases of public health concern due to its zoonotic potential. Its luxurious existence in wild marine fish from an open water body strongly suggests presence of massive sewage pollution. The aim of the current study was to identify and characterize most common streptococci  affecting common pandora (Pagellus erythrinus) inhabiting the Mediterranean coast of Tripoli. A total number of 270 common pandora  were clinically examined for possible streptococcal infection and non-streptococcal infection. The fishes were collected from the area extending from Tripoli to Tajoura (east to Tripoli) during the three seasons, summer, autumn, and spring. No fish samples were available during winter due to the bad climate / storms along the entire western Libyan Mediterranean coasts. Streptococcus iniae, Streptococcus dysgalactiae, Streptococcus phocae, Enterococcus faecalis, and other non-streptococcal species, such as Aeromonas hydrophila, Pseudomonas anguilliseptica, and Photobacterium damselae sub species damselae were biochemically identified. Regardless of the season, S. iniae and E. faecalis were the most prevalent streptococcal species (13% and 8.88%, respectively). In contrast, the most retrieved non-streptococcal species were A. hydrophila, followed by Pseudomonas anguilliseptica, with a prevalence of 10%, and 14.44%, respectively. The highest infections were recorded during autumn, followed by summer, then spring with percentages of 53.34%, 50%, and 43.4%, respectively. The majority of the isolates were sensitive to erythromycin, florfenicol, and sulfamethoxazole-trimethoprim. The molecular screening using the sequences of 16S rRNA genes has confirmed the phylogenetic relationship to S. dysgalactiae, E. faecalis, S. iniae, and S. phocae isolates with similarity percentages exceeding 99.6%. The sequences were deposited in the GenBank with accession numbers (OK033868, OK033869, OK033870, and OK033871). To sum up, the obtained results highly suggests that the common pandora fish from Tripoli coast of the Mediterranean is biologically contaminated with various zoonotic streptococci which could be considered as a biological indicator for municipal sewage pollution.

DOI

10.21608/javs.2024.253995.1299

Keywords

Common pandora, Sewage pollution, south Mediterranean, Streptococci, Tripoli

Authors

First Name

Alaa Eldin

Last Name

Eissa

MiddleName

-

Affiliation

Department of Aquatic Animal Medicine and Management Faculty of Veterinary Medicine, Cairo University Giza 11221, Egypt

Email

aeissa2005@gmail.com

City

Giza

Orcid

0000-0002-7042-8999

First Name

Abdulatif

Last Name

Asheg

MiddleName

A.

Affiliation

Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Libya

Email

a.asheg@uot.edu.ly

City

Tripoli

Orcid

0000-0001-6600-6446

First Name

Abdulsalam

Last Name

Abu Mhara

MiddleName

-

Affiliation

Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Libya

Email

aabumhara@hotmail.com

City

Tripoli

Orcid

-

First Name

Mahmoud

Last Name

Sharaf

MiddleName

-

Affiliation

Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Egypt

Email

sharafmahmoud@cu.edu.eg

City

Prince Edwards

Orcid

-

First Name

Awad

Last Name

Abdelbaky

MiddleName

A.

Affiliation

Department of Aquatic Animal Medicine & Management, Faculty of Veterinary Medicine, Cairo University, Giza11221 Egypt

Email

dr_awad_hr@yahoo.com

City

Giza

Orcid

-

First Name

Amira

Last Name

Attia

MiddleName

S.A.

Affiliation

Department ofVeterinary Publi Health, Faculty of Veterinary Medicine, Zagazig University

Email

dr.attiamirasamir@gmail.com

City

Zagazig

Orcid

-

First Name

Tarek

Last Name

Dakhil

MiddleName

D.

Affiliation

Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Libya

Email

aabsi2003@yahoo.com

City

Tripoli

Orcid

-

First Name

Alkhateib

Last Name

Gaafar

MiddleName

Y

Affiliation

4Hydrobiology Department, Veterinary Research Institute, National Research Centre, Dokki 12622, Giza, Egypt

Email

alkhateibyg@gmail.com

City

Giza

Orcid

-

First Name

Eman

Last Name

Ismail

MiddleName

M.

Affiliation

Department of Veterinary Hygiene and Management, Faculty of Veterinary medicine, Cairo University, Egypt

Email

dr_eman252@cu.edu.eg

City

Giza

Orcid

-

First Name

Rasmia

Last Name

Abu Leila

MiddleName

H.M.

Affiliation

Department of Fish Diseases, Animal Health Research Institute, Agriculture Research Center, Dokki, Egypt

Email

rasmiavet76@gmail.com

City

Giza

Orcid

-

First Name

Heba

Last Name

Abdel Hady

MiddleName

A.

Affiliation

Department of Virology and Serology, Animal Health Research Institute, Alexandria Provincial Laboratory, Alexandria, Egypt

Email

hebaabotier@yahoo.com

City

Alexandria

Orcid

-

First Name

Emad

Last Name

Afify

MiddleName

A.

Affiliation

DSM Nutritional Products Ltd, Wurmisweg 576, 4303 Kaiseraugst, Switzerland

Email

emad.afifi@dsm.com

City

Kafr Elsheikh

Orcid

-

First Name

Abdelbary

Last Name

Prince

MiddleName

-

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt

Email

proteome@cu.edu.eg

City

Giza

Orcid

-

First Name

Reham

Last Name

Ragab

MiddleName

H

Affiliation

Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza 12211 Egypt

Email

reham.hassan@vet.cu.edu.eg

City

Giza

Orcid

-

First Name

Khalid

Last Name

Shahin

MiddleName

-

Affiliation

National Institute of National Institute of Oceanography and Fisheries, Cairo, Egypt

Email

kh.shahin87@gmail.com

City

California

Orcid

-

Volume

9

Article Issue

2

Related Issue

45822

Issue Date

2024-04-01

Receive Date

2023-12-08

Publish Date

2024-04-01

Page Start

31

Page End

41

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 9_Issue 2_Pages 31-41.pdf

PDF . 793.9kB

Link

https://javs.journals.ekb.eg/article_339549.html

Detail API

https://javs.journals.ekb.eg/service?article_code=339549

Order

3

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Biochemical and Molecular Identification of the Most Common Streptococci Affecting Common pandora (Pagellus erythrinus Linnaeus, 1758) From the Mediterranean Coast of Tripoli

Details

Type

Article

Created At

25 Dec 2024