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312343

Assessment of Developmental Competence of Holstein Bulls Spermatozoa upon Addition of Aloe Vera Raw Extract during In Vitro Capacitation

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Theriogenelogy

Abstract

The study aimed to evaluate the effect of the addition of aloe vera raw extract on the capacitation of bovine spermatozoa during the in vitro fertilization process. Hyper activated motility (HAM) and acrosome reaction (AR) of sperm cells, as well as in vitro fertilization and cleavage rates are the main parameters used to estimate the aloe vera's effect on bovine spermatozoa's fertilizing ability. In the current study, two different concentrations of aloe vera (5 and 10 µg/ml) were used. Frozen thawed semen spermatozoa were subjected to a swim-up technique to separate the motile spermatozoa and capacitated in modified Sperm Tyrode's albumin lactate pyruvate (S-TALP) medium supplemented with heparin only without aloe vera treatment (positive control), heparin+5µg/ml aloe vera, heparin+10µg/ml aloe vera, and finally the negative control tube, which was supplemented with 5µg/ml aloe vera without adding heparin. Sperm cells were incubated for 90 minutes at 39C0 in a 5% CO2 incubator and evaluated every 30 minutes at intervals. Cumulus oophorus complexes (COCs) were matured in a 5% CO2 incubator at 39C0 and inseminated in vitro with frozen-thawed bull sperm at the above concentrations. The inseminated oocytes were incubated at 39C0 in a 5% CO2 incubator for 24 hours and then examined for evidence of fertilization. The current study revealed that the aloe vera concentration of 10µg/ml at incubation time (60 minutes) had the best effect on sperm capacitation and acrosome reactions. The overall percentage of spermatozoa with progressive motility (PM) had declined across all groups as time progressed, with a significantly lower value found at the above concentration, as well as the effect of sperm treated with aloe vera on fertilization rate. The results showed that the proportion of fertilized oocytes was significantly increased at the aloe vera concentration of 10µg/ml compared to the other concentrations (5µg/ml), positive control, and negative control, respectively. In conclusion, treatment of bull spermatozoa with 10 µg of aloe vera extract/ml semen was considered the most efficient concentration for enhancement of sperm capacitation as a high IVF rate was obtained in Holstein Bull.

DOI

10.21608/javs.2023.219422.1249

Keywords

Aloe vera, Capacitation, Holstein bull spermatozoa, in vitro fertilization

Authors

First Name

Mona

Last Name

Mahmoud

MiddleName

-

Affiliation

Theriogenology Department, faculty of veterinary medicine, Beni suef university, Beni suef, Egypt

Email

monamahmoud@vet.bsu.edu.eg

City

Beni suef

Orcid

0009-0009-8458-3111

First Name

Saber

Last Name

Abd-allah

MiddleName

-

Affiliation

Theriogenology Department, faculty of veterinary medicine, Beni suef university, Beni suef, Egypt

Email

saber@vet.bsu.edu.eg

City

Beni suef

Orcid

-

First Name

Abdel-Halim

Last Name

B.R.

MiddleName

-

Affiliation

Theriogenology Department, faculty of veterinary medicine, Beni suef university, Beni suef, Egypt

Email

drbakarwa@yahoo.com

City

Beni suef

Orcid

-

First Name

Khalil

Last Name

A.A.Y.

MiddleName

-

Affiliation

Theriogenology Department, faculty of veterinary medicine, Beni suef university, Beni suef, Egypt

Email

abdeltawab.yaseen@vet.bsu.edu.eg

City

Beni suef

Orcid

-

Volume

8

Article Issue

4

Related Issue

42650

Issue Date

2023-10-01

Receive Date

2023-06-22

Publish Date

2023-10-03

Page Start

43

Page End

53

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 8_Issue 4_Pages 43-53.pdf

PDF . 702.6kB

Link

https://javs.journals.ekb.eg/article_312343.html

Detail API

https://javs.journals.ekb.eg/service?article_code=312343

Order

7

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Assessment of Developmental Competence of Holstein Bulls Spermatozoa upon Addition of Aloe Vera Raw Extract during In Vitro Capacitation

Details

Type

Article

Created At

25 Dec 2024