Lumpy skin disease virus (LSDV) is a member of the genus Capripox virus within the family Poxviridae that infects cattle causing unneglectable economic losses. Providing a suitable cell culture for virus propagation is essential goal to be used for virus isolation and vaccine production. The present work deals with a novel cell culture that is the ovine lamb heart (OLH) to investigate its benefit for LSDV propagation in comparison with the use of African green monkey kidney cells (Vero) and Madin Darby bovine kidney (MDBK) cell cultures. Ten serial passages of Lumpy skin disease virus in each cell culture revealed similar cytopathic effect recorded the peak virus titer (6.0, 5.5 and 5 Log10 TCID50/ml in OLH, Vero and MDBK cell cultures respectively) by the 6th day post cell infection the time which determined to harvest the highest titer by studding the virus growth curve in each cell culture. Virus neutralization test (VNT) and direct fluorescent antibody technique (FAT) using specific anti-LSDV sera confirm the presence of all used cell cultures. So, it could be concluded that OLH cell culture represents a suitable and best one for propagation of LSDV and more researches are in need to evaluate its use for vaccine preparation.