Beta
ManageSearchChatDiscover
287168

Detection and Molecular Characterization of Some Virulence Genes of Escherichia Coli Isolated from Milk in Dairy Cow Farms

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Bacteriology

Abstract

Coliform pathogens, primarily E. coli, were discovered throughout the farm, causing environmental mastitis and can be shed from the udder into the milk; they are concerned about severe gastrointestinal disruption and potential enteropathogenic and/or toxic strains, posing a risk to public health. The objectives of this study were to identify the incidence and harmful serotypes of pathogenic Escherichiacoliand some of their virulent genes, which were isolated from the collected milk of some dairy farms in the Delta region and Cairo-Alexandria desert road farms, in Egypt during one year using the polymerase chain reaction (PCR) technique after bacteriological and serological identification as well as determine the antimicrobial susceptibility of the isolated strains. 150 milk samples in total were gathered (100 milk samples from bulk milk tanks and the other 50 samples from clinically mastitic dairy cows). According to our finding, the mean values of somatic cell count (SCC), standard plate count (SPC), and coliform count (CC) in the hundred bulk tank milk samples were 3.67  1.08×104/ml, 7.08×104± 6.25×104 cfu/ml and 3.04×102±1.43×102/ml, respectively. The bacteriological investigation exhibited that, the Escherichiacoli incidences from bulk tank milk (BTM) and mastitic milk samples (MMS) were 12% and 18%, respectively. The detected   E.coli serotypes including, O26, O44, O55:K99, O111, O119 and O157:H7 from MMS, while O1, O55, O78, O86, O124 and O158:H10 from BTM. Molecular virulence characterization of E.coli strains showed that, Shiga toxins 2 (stx2) gene is present in O157:H7, while the stx1 gene present in O26.  The Intimin gene (eaeA) is involved in four strains, O44, O111, O119, and O157:H7. Positive amplification of a biofilm gene (adrA) appeared in all E.coli strains. The outcome of the antimicrobial susceptibility revealed high resistance to amoxicillin (85.71%), streptomycin (80.95%), ampicillin (71.43%), and flucloxacillin (61.90%). Meanwhile, the highest susceptibility was to ciprofloxacin (95.24%) followed by enrofloxacin (90.48%), neomycin (80.95%), and gentamycin (76.19%). Effective hygienic measurements are required to avoid toxigenic and pathogenic E.coli and more future studies should be performed to increase awareness in dairy farms.

DOI

10.21608/javs.2023.173140.1190

Keywords

Antimicrobial susceptibility, E.coli, mastitis, PCR, STEC, Virulence genes

Authors

First Name

Ola

Last Name

Abd El-Fattah

MiddleName

A.

Affiliation

Giza Lab., Animal Health Research Institute, Giza, Agricultural Research Center (ARC), Egypt

Email

ola_72vet@yahoo.com

City

-

Orcid

-

First Name

Ebtsam

Last Name

Kotb

MiddleName

E.Z.

Affiliation

Udder Health and Neonatal Diseases, Animal Reproduction Research Institute, Giza, Agricultural Research Center (ARC), Egypt

Email

dekotb@yahoo.com

City

-

Orcid

-

First Name

Hala

Last Name

Ibrahim

MiddleName

S.

Affiliation

Serology Unit, Animal Health Research Institute, Giza, Agricultural Research Center (ARC), Egypt

Email

-

City

-

Orcid

-

First Name

Amany

Last Name

El Gohary

MiddleName

H.

Affiliation

Serology Unit, Animal Health Research Institute, Giza, Agricultural Research Center (ARC), Egypt

Email

-

City

-

Orcid

-

First Name

Abeer

Last Name

Mohammed

MiddleName

A.E.

Affiliation

Buffalo Diseases Research Department, Animal Health Research Institute, Giza, Agricultural Research Center (ARC), Egypt

Email

-

City

-

Orcid

-

Volume

8

Article Issue

2

Related Issue

40526

Issue Date

2023-04-01

Receive Date

2022-12-13

Publish Date

2023-04-01

Page Start

1

Page End

9

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 8_Issue 2_Pages 1-9.pdf

PDF . 601.7kB

Link

https://javs.journals.ekb.eg/article_287168.html

Detail API

https://javs.journals.ekb.eg/service?article_code=287168

Order

1

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Detection and Molecular Characterization of Some Virulence Genes of Escherichia Coli Isolated from Milk in Dairy Cow Farms

Details

Type

Article

Created At

25 Dec 2024