Background: A new reality mandates that adopting electronic information systems (EIS) is recognized as essential for advancing nursing processes and improving the quality of patient care. However, Egyptian health organizations have paid little attention to adopting health information systems, resulting in inadequate staff preparedness as a challenge to EIS compliance and application. Objectives: This study aimed to assess nurses' readiness to use the electronic information system. Material and Methods: A descriptive cross-sectional study was conducted at the National Cancer Institute (NCI) in Egypt, involving a convenience sample of nursing staff (n = 402) willing to participate. The nurses completed a self-administered questionnaire as part of the study. Results: Among the 402 nurses who participated in the survey, more than half (53.2%) were female. The mean age was 32.55 ± 10.47 years, with an age range of 21 to 58 years. The majority of the participants (54.0%) held a technical diploma. The overall readiness for EIS was 76.6%. Most study participants (81.27%) exhibited a positive attitude toward using EIS. In comparison, most (72.5%) highly perceived EIS usability and demonstrated acceptable computer skills (74.69%). Conclusion: Overall, the readiness among nurses is encouraging, and their highly positive attitudes and perceptions toward EIS suggest that the implementation of EIS would be welcomed at NCI.