This study was conducted to investigate the therapeutic effects of Nigella sativa on steatohepatitis disease in diabetic (SDM) rats. Fifty rats were separated into two main groups as follows. The first main group (N=10), and the second main group (N=40). The first group was fed on a basal diet and kept as the negative control group, while the other rats were fed on basal diet Deficient in Methionine- and Choline for 6 weeks, after the induction of steatohepatitis, the rats were injected with streptozotocin at dosages of (60 mg/kg body weight) to induce diabetes. Then, rats were reclassified into four equal groups: subgroup one served as the control positive group and three treated rat subgroups were fed on basal diet supplementation with (5, 7.5 and 10%) of N. Sativa seeds powder, respectively. Results showed that N. Sativa contain high amounts of carbohydrate and protein while, low amount of fat. It is also rich in phenolic and flavonoids compounds which are considered antioxidants. Results revealed that N. Sativa with the three different levels had improved of body weight accompanied by a significant decrease in levels of glucose, insulin, liver functions (ALT, AST and ALP), as well as in lipid profile, while were recorded a significant increase in a high-density lipoprotein-cholesterol (HDL-C). In addition, significantly reduced malondialdehyde (MDA) while the antioxidants enzymes glutathione (GSH) was significantly (P<0.05) increased compared to untreated steatohepatitis diabetic rats feed on the basal diet alone. N. Sativa could introduce a potential natural therapy against steatohepatitis diabetics.