This study attempted to examine the impact of underlying processes of lemon balm leaves (Melissa officinalis L.) on rats with acute kidney injury (AKI) provoked by glycerol.  Thirty male albino rats were utilized and weighed 170 ± 5g and divided into five groups (six rats of each). Group 1 was maintained on the standard basal diet and served as a negative control group. Groups 2, 3, 4 and 5 were administered glycerol (10 ml/kg b wt., fifty percent v/v in sterile saline, i.p.) to induce AKI. Group 2 was fed on the basal diet and kept as a positive conrol group, while the others three groups fed on the supplemented basal diet with 5, 10 and 15% lemon balm leaves, respectively. The findings indicated that treated AKI groups with lemon balm leaves (5%, 10%, and 15%) have variations in BWG, feed efficiency ratio & feed intake. As well, there is a significant decrease (P < 0.05) in liver and kidneys weights of treated AKI groups with lemon balm leaves, compared with that of the positive control group. The positive control group had significantly higher levels of liver functions (ALT, AST & ALP), lipid profile (cholesterol, triglycerides, LDL-c, VLDL-c), kidney functions (uric acid, blood urea nitrogen & creatinine), malondialdehyde and glutathione and decrease in HDL-c, comparison to the treated AKI groups with the different levels of lemon balm leaves. Accordingly, lemon balm was suggested in this investigation to affective in acute renal disorders.