This study aimed to investigate the effects of different levels of selenium-enriched Saccharomyces cerevisiae (S. cerevisiae) on the production performance, egg quality, lipid profile, antioxidant status, and intestinal health of laying hens under hot environmental conditions. A total of 200 healthy 23-wk-age Brown Lohmann layers were randomly divided into 4 treatments, each in 5 replicates as follows: the first group (Se-ES0) fed the basal diet without supplementation (control), the second group fed the basal diet supplemented with 0.25 g selenium-enriched S. cerevisiae (Se-ES1), the third group fed the basal diet supplemented with 0.5 g selenium-enriched S. cerevisiae (Se-ES2), the fourth group fed the basal diet supplemented with 1.0 g selenium-enriched S. cerevisiae (Se-ES3). After 8 weeks, the results showed a deterioration in the productive performance and egg quality of laying hens fed on a diet without additives under heat stress conditions (P < 0.05). Compared with the control group, the feed conversion ratio (FCR) improved (P < 0.05), and the egg production%, albumen weight, albumen height, and Haugh unit in the Se-ES2 and Se-ES3 groups increased significantly (P < 0.05). Furthermore, the antioxidant capacity in the Se-ES3 group was enhanced than that in the other groups (P < 0.05) decreased MDA and increased SOD levels. Additionally, the lipid profile improved in the Se-ES3 group (P < 0.05) by decreased cholesterol and increased HDL levels. Moreover, supplementation with Se-ES modified the microbial content, as the Lactobacillus count increased, and the E. coli and Coliform count decreased. It can be concluded, that adding selenium-enriched S. cerevisiae improved the egg production%, FCR, and Haugh unit and enhanced microbial community, lipid profile, and antioxidant status of heat-stressed laying hens.