The aim of the present study was to evaluate the seasonal variations in sperm quality of APRI Line and Baladi Black Rabbit Bucks kept under Egyptian climate. Semen ejaculates from 20 rabbit bucks of APRI and Baladi Blacks (BB) in winter, spring, autumn and summer were analyzed. Sperm quality parameters such as kinematics, morphology and DNA integrity were analyzed and study the effect of climate factors recorded by the web of local meteorological office. There were significant differences between ARRI and BB for ejaculate volume (0.59, 0.55 ml), live sperm per ejaculate (83.54, 79.34%), progressive motility (58.00 and 60.00 %), comet length (37.06 and 38.34 px) comet height (34.18 and 37.74 px) and head diameter (33.54 and 36.53 px), respectively. Whereas, non-significant differences were observed due to the effect of line on semen pH, sperm abnormalities per ejaculate, tail length and DNA % in tail. The effect of season was significant for ejaculate volume (0.64 and 0.48ml), live sperm per ejaculate (84.10 and 78.20 %), progressive motility (76.00 and 45.00%), sperm abnormalities per ejaculate (11.52 and 16.20%), comet length (36.64 and 38.76), tail length (2.55 and 5.06 px) and DNA% in tail (6.17 and 4.26%) during winter and summer seasons, respectively while, non-significant differences were observed between seasons for semen pH, comet height and head diameter. The results showed that the highest ejaculate volume and live sperm per ejaculate were recorded during winter with APRI moreover; sperm abnormalities per ejaculate in summer season were lower for APRI (14.60 %) than for BB (17.80 %). It is concluded that, APRI line rabbit bucks tended to have larger ejaculate volume, lower semen pH, higher total live sperm, higher progressive motility percentage and lower abnormal percentage than those displayed by the BB rabbit bucks this, could be an indicator to an acceptable line that performs well under Egyptian conditions.