This study aimed to examine the impact of probiotic treatment (ZAD) on in vitro dry matter digestibility (IVDMD), gas generation, and detoxification of corn silage (CS) contaminated with aflatoxins (5ppb). The samples were divided into 7 different groups whereas control (C) contaminated sample, T1: C+ 1ml ZAD/ Kg DM, T2: C+ 1ml ZAD/ Kg DM, T3: C+ 2ml ZAD/ Kg DM, T4: C+ 2ml ZAD/ Kg DM, T5: 3ml ZAD/ Kg DM for and T6: C+ 3ml ZAD/ Kg DM. T1, T3 and T5 were incubated for one week after adding ZAD, while T2, T4 and T6 incubated for two weeks. Measurements were taken for aflatoxin, in vitro dry matter digestibility (IVDMD), gas generation, Ruminococcus bacterial count, and chemical analysis. The crude protein (CP) values for all treatments (7.3, 7.6, 9.9, 10.1, 10.3 and 10.9% respectively) were considerably greater than the control (3.2%). The crude fiber (CF) levels in all treatments (24.4, 24, 23, 22.5, 21.5 and 18% respectively) were significantly lower than the control (28.5%). Evaluation of (IVDMD) occurred at 6, 24 and 48 hours. The control group had percentages of 31%, 35.5%, and 42% at each period. T1 had percentages of 33%, 36%, and 44.7%. T2 had percentages of 33.4%, 45%, and 42.7%. T3 had percentages of 36.5%, 46.6%, and 54%. In vitro dry matter disappearance was increased gradually by treatment at all time points. This indicates that the application of ZAD treatment significantly improves the in vitro dry matter digestibility (IVDMD). Notable variations in gas generation were seen among the control group and all treated groups at different time points of IVDMD. Probiotic ZAD effectively eradicates aflatoxin at a concentration of 3 ppb in T6.