In the current study, growth performance, intestinal morphology, and digestive enzymes activity for broilers challenged with aflatoxins (AF) were examined to determine the preventive efficacy of food supplementation with tomato waste powder (TWP). A total of 300 Ross (308) broiler chicks one-day old were randomly distributed in five treatments and six replicates (ten birds per replicate); feed and water were provided ad libitum throughout the 35 days experiment. Treatments were as follows: (T1) A basal diet containing neither AF nor TWP (positive Control) and (T2) a basal diet containing 100 µg/kg AFB1 (negative control). The other three treatment groups were supplemented with TWP at 2.5, 5.0 and 7.5 g/kg plus the 100 µg/kg AF. The results showed that AFB1 decreased growth parameters, diminished the intestinal villus height (VH) and crypt depth ratio (VCR) while raising the crypt depth (CD), decreased (p<0.05) intestinal amylase and lipase activity and increased pathogenic bacteria compared to the control group.  On the other hand, dietary TWP supplemented diets alleviated the adverse effects of AF on growth and gut parameters. Dietary TWP supplementation (5.0 and 7.5 g/kg) resulted in significantly improved (p<0.05) growth performance, intestinal amylase and lipase activity with AFB1 contaminated diet. Additionally, decreased the pathogenic bacteria. These findings suggested that TWP is a promising feed supplement in the broiler industry.