In the present study, African catfish Clarias gariepinusis one of the most important cultured fish in tropical and subtropical regions and is an economically important fish species, the need to reduce cost of feeding, become more popular among world fish farmers and drive to increase fish productivity that motivated the present research. The present practical study was conducted out at the experimental fish farm, fish production department, faculty of agriculture, Al-Azhar University, Cairo, Egypt. The experimental period (98 days, 14 weeks) started during first 1^thAbril to 15^rd July 2023. A 98-day feeding trial was conducted in four tanks system has consist of a four series of rectangle concrete tanks (in each divided two basins). 240  C. gariepinus post fingerlings (Sixty experimental fish in each treatment ) each of average initial weight 38.87±1.14 - 39.75±1.19 g and Initial Fish length 19.2±1.07 - 20.1±1.12 (cm) to check the effect of graded quantitative levels of partial replacement of local Basaria ( Sand Smelt ) meal (SSM) and Biogen as a main source of protein with chitosan nanoparticles biosynthesis and powder Spirulina platensis in diets for African catfish ( clarias griepinus ) reared in tanks , on water quality, growth performance , feed utilization ,chemical body compositions parameters. Four experimental diets were prepared by T1, at a rate of 0% (control, basal diet, contain40% crude protein, local Sand Smelt meal and Biogen), T2 (chitosan nanoparticle biosynthesis(CSNPs) of 10 g kg^-1 and powder of S.platensisas a rate of 50 g kg^-1, T3(CSNPs) of 20 g kg^-1 and 100 g kg^-1powder of  S.platensis, and T4(CSNPs)of 30 g kg^-1 and 150 g kg^-1 powder of S.platensis, respectively .These were replicated twice.  Chitosan nanoparticles biosynthesis (20 g kg^-1) and powder Spirulina platensis (100 g kg^-1) in diets for C. griepinus reared in tanks was found to significantly (P<0.05) the best level improved water quality daily weight gain, survival, feed utilization and meat quality as well as body composition (P<0.05) of C. gariepinus post fingerlings which lay waste globally has the potentials to revolutionize aquaculture