The present study aimed to evaluate the effect of zeolite addition to rations on liver and kidney functions, minerals profile and aldosterone hormone of Barki ewes affected by saline well water throughout gestation period in South Sinai, Egypt. Thirty Barki ewes (2.0-3.0 years old and 31.8 ± 1.4 kg average body weight) were randomly assigned into three equal groups (10 ewes / group). The 1^st group (G1); ewes drank fresh (TW) tap water (274 ppm TDS) and fed the basal diet (control group). The 2^nd group (G2) ewes drank saline (SW) well water (5980 ppm TDS) and fed the basal diet withot zeolite addition. The 3^rd group (G3) ewes drank SW (5980 ppm TDS) and fed the basal diet supplemented with 60 g zeolite /kg diet (6 %). Rations were adjusted monthly to cover their nutritional requirements during the experimental period. Fresh and saline water were available freely all the day time. Results indicated that Alanine amino transferase (ALT) and aspartate amino transferase (AST) concentrations were significantly higher in saline group (G2) then G3 and control group (24.41, 19.63 and 16.29 IU/dl, respectively) and (34.19, 29.42 and 25.26 IU/dl) respectively. Urea and creatinine concentrations tended to increased significantly in saline group (G2) followed by zeolite group (G3), while control group recorded the lowest value. P and K concentrations were significantly (P˂0.05) higher in control group than others, while Ca significantly decreased in control as compared to other groups. Cl and Na were insignificantly higher in G2 than other groups. Aldosterone concentrations were affected by drinking saline water.  Both G2 and G3 recorded 24.1 and 17.5% reduction in aldosterone concentration at 140 day of pregnancy compared to control group. In conclusion, addition of zeolite at a level of 6 % to the diets of Barki ewes could be an attempt to reduce the negative effect of drinking saline water.