Background: The primary golden aim of fetal well-being assessment is reducing the 
incidence of fetal distress, perinatal morbidity and mortality. Nurses play a critical role 
in the assessment of fetal well-being through application and interpretation of fetal well
being assessment methods. The aim of this study was to evaluate the effect of the 
educational guidelines regarding assessment of fetal well-being during pregnancy on 
nurses' performance. Subjects and method: A quasi experimental research design was 
used. It was conducted at Antenatal, Ultrasound and Fetal Medicine unit at obstetric and 
gynecological department of Tanta University Hospital. The total study sample was all 
available nurses (60). Two tools were used for collection of data. Tool (I): Nurses' 
knowledge regarding assessment of fetal well-being during pregnancy: It included 
two parts: Part (1): Socio demographic characteristics of nurses and Part (2): Nurses' 
knowledge regarding fetal well-being assessment during pregnancy. Tool II: 
Assessment of fetal well-being observational checklist: It included two parts: Part 
(1): Nurses' practices regarding non-invasive procedures and Part (2): Nurses' practices 
regarding invasive procedures. Results: The mean knowledge score and mean practices 
score of nurses regarding fetal well-being assessment during pregnancy were increased 
immediately and three months after implementation of the educational guidelines with a 
statistical significant difference. Conclusion: The research hypothesis has been 
achieved which resulted in a statistically significant positive improvement of nurses' 
performance immediately and three months after implementation of the educational 
guidelines. Recommendations: Evident and continuous educational programs are to be 
established at hospitals for nurses to improve their awareness and practices regarding 
fetal well-being assessment during pregnancy.