Background: Umbilical venous catheter (UVC) is very easy and quick method of gaining 
access to deep vein in comparison to cannulation. Aim: The research aim was to evaluate the 
effect of implementing educational program about care for neonates undergoing UVC 
installation on nurses' performance. Design: A quasi-experimental research design was 
utilized; the research was conducted at neonatal intensive care unit (NICU) El-Mahalla 
General Hospital. Subjects and Method: A convenience sampling of sixty nurses who were 
working at above mentioned setting and forty neonates who were admitted at NICUs within a 
period of six months whose umbilical cord were still connected to circulatory system and 
both sexes. Tools of data collection: Tool (I): Structured interview schedule: It includes a: 
Socio demographic characteristic of nurses b- Neonates' characteristics and Nurses 
knowledge about care of neonates undergoing UVC installation. Tool (II) An observational 
Check list to assess nurses' practice. Results: There were a positive correlation between 
nurses′ knowledge and their practices before, immediately after program implementation and 
one month after program implementation regarding care of neonates undergoing UVC 
installation. Conclusion: Nurses′ knowledge and practice were improved after implementing 
the program. Recommendation: The pediatric nurse should be taught the basic clinical skills 
regarding care of UVC periodically and regularly through applying a training program.