An inactivated virus vaccine (MDBK-dextran) containing strains of bovine viral diarrhoea (BVD), infectious bovine rhinotracheitis (IBR), parainfluenza type 3 (PI-3) and bovine adeno virus type-3 was conven­iently produced by propagation on MDBK-dextran-roller system, then, the viruses were inactivated with binary ethyleneimine (BEI), lower con­centration of inactivants which killed the viruses in the shortest period of time was selected for preparing the corresponding vaccine. Alhydrogel was added in a final concentration of 30% to the vaccine as adjuvant. Ef­fectiveness of each fraction of the prepared vaccine was studied in chal­lenged immunity studies. Cattle vaccinated with any fraction of vaccine followed by challenge with a disease causing strain of that fraction, re­sisted infection well by both forms; qualitative (clinical aspect) and quan­titative points of view (virus isolation and temperature). All antigens stimulated high antibody titres detected by ELISA immuno assay (DA) and serum neutralization test (SNT) against all component of the vac­cine and haemagglutination inhibition test (HIT) aganist P1-3. Immunity obtained was long-lasting and even persisted up to 6 months post-vaccination.