Plants with medicinal properties are a vital global source of medications that can save lives. The most important instrument for choosing, propagating, and preserving the rare and important species of medicinal plants is biotechnology. Because it contains multiple types of secondary metabolites, Butea monosperma has vast therapeutic capabilities that have earned it a prominent place in the pharmaceutical industry. The minimal seed viability, low germination of the seeds rate, and genetic heterogeneity of B. monosperma impede its propagation. The main obstacles to the long-term cultivation of this significant plant are overexploitation, significant habitat damage, and limited ranges. Butea monosperma is a prominent medicinal plant, and it's in vitro tissue cultivation and micro-propagation are well-established processes. For this particular plant species, the fast and repeatable in vitro response to treatments with plant growth regulators has become a crucial component of genetic transformation research. This chapter covers advances and improvements in the genetic transformation of B. monosperma as well as methods for in vitro regeneration. In conclusion, we offer recommendations and future directions for this significant species of tree with medicinal value.