Nine fungi were isolated from naturally infected grains of all sor­ghum varieties used in this study. Out of these fungi, two namely; Aspergilus flavus and A.parasiticus are well known to produce aflatoxins. The aflatoxin 131 was detected in the grains of samples obtained from 6 sorghum varieties ranging from traces to 11.2 ug/kg grains, while the aflatoxin B2 was found in grains of 3 sorghum varieties in concentrations ranging from 3.4 to 11.2 ug/kg grains. On the other hand, the aflatoxins 61 and G2 were not detected in the naturally infected grains of all sor­ghum varieties tested. Following artificial inoculation with A.flavus and A.parasiticus, indi­vidually, in plants of cv. Giza 15, the four aflatoxins 81, B2, G1, and G2 were produced in the grains with high concentration, when compared with aflatoxins 131 and 132 produced in the naturally infected grains. Grains inoculated with A.parasiticus produced the highest amount of af­latoxin G1(50 ug/kg), while grains inoculated with A.flavus produced the highest amount of the aflatoxins 131, B2 and G2 (maximum 20,20 and 25 ug/kg, respectively).