A field experiment was conducted in a calcareous soil, poor in available DTPA extracted Fe (6.27 ppm) and Mn (5.36 ppm), at Noubar­ia Reseasch staion Farm to investigate the effect of spraying with Fe (50 or 100 ppm Fe as FeSO4 or Fe-EDDHA) and /or Mn (50ppm Mn as MnSO4) on root yield, root dry weight, nutrients uptake of N, P, K, Fe, Mn and Zn, total carbohydrate, and total soluble solids (T.S.S.) in sugar beet roots. The results emphasized that the amount of root yield, root dry matter and nutrients uptake were significantly higher in the com­bined treatment (Fe x Mn) than the other treatments and reached a maximum with Fe-chelated form. Little increase in carbohydrates was found by Fe and/or Mn addition. The interaction effect of Mn and Fe was significant in raising T.S.S. (T/fed.).