Edible green algal species are the most widely distributed and diverse macroalgae in the world and are considered as an important source of bioactive molecules which serve as multi-product sources for nutritional and pharmaceutical applications. The present investigations were carried out concerning biochemical constituents from three species of green marine algae Ulva conglobata, Caulerpa racemosa, and Bryopsis plumosa collected from the coast of Veraval Chowpati, Gujarat India. The biochemical composition was analysed using uv - spectrophotometer to evaluate its food value and find out variations in composition during the investigation period. The reducing sugar, proline, and starch contents were high in Bryopsis plumosa followed by Caule rpa racemosa and Ulva conglobata. Proline content is higher than the total amino acids in three algal species. The protein contents following by Lowry method Caulerpa racemosa have a high amount with 1667.32 ± 18.42 (µgg-1 dry wt.) followed by Bryopsis plumosa 1394.98 ± 18.78 (µgg-1 dry wt.) and Ulva conglobata 292.72 ± 17.85 (µgg-1 dry wt.). The protein content was recorded maximum in Bryopsis plusmosa and Caulerpa racemosa than in the Ulva conglobata. All the assays carried out in the present work showed that all selected green algae are good sources of biochemical. According to the biochemical composition values of the studied algae, they have the potential to be a source of constituents with high nutritional value and use in the food, dietary, and pharmaceutical industries.