A total of 110 serum samples, as well as, 110 milk samples were collected from breeding cattle at different localities at lsmaelia Governorate. Samples were examined for the presence of bovine viral diarrhoea (BVD) virus antibodies using the SVANOVIR-kit by indirect ELISA technique. The results showed that, 49.1% of sera and 50% of milk saamples were positive for BVDV antibodies. Milk is preferable to blood in large scale epidemiological surveys, since the sampling procedure is much simpler, by using ELISA technique.